Abstract：Objective：To discuss the potential mechanism of Huanshao Pellets in treating oligonasthenospermia based on network pharmacology and molecular docking. Methods： The effective components of Chinese medicinals were obtained by TCMSP and TCMIP. The GEO database was used to search for desease-related genes， and the intersection analysis of Chinese medicinal genes and disease genes was carried out. The PPI protein interaction network was constructed via STRING platform. The common genes were analyzed by Cytoscape software， and the intersection genes were analyzed by GO function and KEGG pathway by R software. The molecular docking software such as AutoDock Tools and QuickVina-W was used to verify the accuracy of the analysis results. Results： The research obtained 195 active components， 1 101 active genes， 108 medicinal-disease intersection targets， and 8 core pharmacodynamic molecules，including stigmasterol，quercetin，daidzein，denudatin B，kaempferol，asperuloside tetraacetate，suchilactone，ergosterol peroxide. The key targets were CDC25A，TOP2A，AURKA，CHEK1，PLK1， KIF11， PLK4 and TTK. GO function results showed that the biological processes of the treatment of oligonasthenospermia by Huanshao Pellets were mainly concentrated in phosphorylation，regulation of kinase activity， steroid metabolism， lipid metabolism and hormone metabolism. KEGG pathway was mainly enriched in calcium signaling pathway，Rap1，Ras，MAPK，PI3K-Akt，AMPK，FoxO signaling pathway，cell cycle，exocytosis and so on. Molecular docking showed that the core pharmacodynamic components had good docking activity with the predicted action targets. Conclusion： The mechanism of action of Huanshao Pellets in treating oligoasthenospermia may be through the pharmacodynamic molecules such as stigmasterol， quercetin and daidzein， through the calcium signaling pathway， Rap1， Ras and other signaling pathways， acting on the key targets such as CDC25A， TOP2A， AURKA， and through multiple biological processes such as phosphorylation， kinase activity regulation， and steroid metabolism. Finally，the effect of treatment of oligoasthenospermia is achieved.