Abstract：Objective：To observe the effects and potential mechanisms of Astragalus polysaccharides （APS） on the gastric cancer （GC） cell line HS-746T. Methods： The binding of microRNA-25 （miR-25） to FBXW7 was predicted using the TargetScan tool，and the relationship between miR-25 and FBXW7 was determined using a dualluciferase reporter assay. Human gastric cancer cell line HS-746T cells were cultured in vitro and divided into the following groups based on experimental needs： the control group （transfected with blank control gene）， inhibitor group （transfected with miR-25 inhibitor）， APS group （transfected with blank control gene and cultured in DMEM medium containing 1 mg/mL APS for 24 hours）， miR-25 mimics group （transfected with miR-25 overexpression gene），and APS+miR-25 mimics group （transfected with miR-25 overexpression gene and cultured in DMEM medium containing 1 mg/mL APS for 24 hours）. The expression levels of miR-25 mRNA and FBXW7 mRNA in each group were detected by real-time quantitative PCR；cell proliferation was measured by MTT assay，and cell invasion ability was assessed by Transwell assay. Results：The Starbase tool predicted the binding of miR-25 to FBXW7，and the dualluciferase reporter assay revealed a direct binding relationship between miR-25 and FBXW7. Compared with those in the control group，the miR-25 mRNA expression level decreased and the FBXW7 mRNA expression level increased in the inhibitor group （P＜0.05）；miR-25 mRNA expression decreased and FBXW7 mRNA expression increased in the APS group （P＜0.05）； miR-25 mRNA expression increased and FBXW7 mRNA expression decreased in the miR-25 mimics group （P＜0.05）. Compared with those in the miR-25 mimics group，miR-25 mRNA expression significantly decreased and FBXW7 mRNA expression significantly increased in the APS+miR-25 mimics group （P＜0.05）. MTT assay results showed that after 48 hours， the cell proliferation rate in the inhibitor group was lower than that in the control group （P＜0.05），and the cell proliferation rate in the miR-25 mimics group was higher than that in the control group （P＜0.05）. Transwell assay results showed that the number of transmembrane cells in the inhibitor group was less than that in the control group （P＜0.05）， and the number of transmembrane cells in the miR-25 mimics group was greater than that in the control group （P＜0.05）. Conclusion：APS can inhibit the proliferation and invasion of HS- 746T cells， inhibit the expression of miR-25 mRNA， and promote the expression of FBXW7 mRNA. APS can also reverse the increase in miR-25 and the inhibition of FBXW7 caused by cell transfection with miR-25 mimic. APS can inhibit the proliferation and invasion of GC cells HS-746T through the miR-25/FBXW7 signaling pathway.