Abstract： Objective： To observe the clinical effect of the microneedle knife combined with acupuncture to regulate the mind in the treatment of female stress urinary incontinence (SUI) based on the brain-heart-kidney theory. Methods： A total of 90 cases of female SUI patients were selected and randomly divided into three groups：the microneedle knife and acupuncture group，the acupuncture group， and the pelvic floor muscle rehabilitation group， with 30 cases in each group. Clinical effects were compared among the three groups. The changes in 1-hour urine leakage volume， 72-hour urine leakage frequency，and the scores of the Depression Anxiety Stress Scales-21 (DASS-21) were compared among the three groups before and after treatment. Results：The total effective rates in the microneedle knife and acupuncture group， the acupuncture group， and the pelvic floor rehabilitation group were 96.67%， 83.33% ， and 73.33% ， respectively ， the difference being significant tested by chi-square test (P ＜ 0.05). After treatment ， the 1-hour urine leakage volume in the three groups was decreased when compared with that before treatment (P＜0.05)；the 1-hour urine leakage volume in the microneedle knife and acupuncture group was less than that in the acupuncture group and the pelvic floor rehabilitation group (P＜0.05)， while there was no significant difference between the acupuncture group and the pelvic floor rehabilitation group (P＞0.05). After treatment， the 72-hour urine leakage frequency in the three groups was decreased when compared with that before treatment (P＜0.05)， and the 72-hour urine leakage frequency in the microneedle knife and acupuncture group was less than that in the acupuncture group and the pelvic floor rehabilitation group；there was no significant difference between the acupuncture group and the pelvic floor rehabilitation group (P＞0.05). After treatment， the scores of anxiety， depression and pressure，as well as the total scores in the three groups were reduced when compared with those before treatment (P＜0.05)， and the above scores in the microneedle knife and acupuncture group and the acupuncture group were lower than those in the pelvic floor rehabilitation group (P＜0.05)； there was no significant difference between the microneedle knife and acupuncture group and the acupuncture group (P＞ 0.05). Conclusion： The combination of the microneedle knife for release and acupuncture to regulate the mind has a more significant therapeutic effect on female SUI， effectively reducing the amount and frequency of urinary leakage，and improving mental and emotional well-being.