Abstract： Objective： To observe the clinical effect of the combination use of Jin's three-needle therapy and virtual reality (VR) technology on improving the upper limb motor function of patients with stroke complicated with hemiplegia based on routine therapy. Methods： A total of 72 patients with stroke complicated with hemiplegia were divided into the control group and the observation group by the random number table method， with 36 cases in each group. Both groups were treated with basic medicines and routine rehabilitation. The control group was given VR intelligent exercise training， and the observation group was given VR intelligent exercise training combined with Jin's three-needle therapy. Both groups were treated for four weeks. The scores of Fugl-Meyer Assessment (FMA) scale and Modified Barthel Index (MBI) scale before and after treatment were evaluated； the root mean square amplitude (RMS) of biceps brachii and triceps brachii on the affected and healthy sides was detected，and the RMS ratios of the affected sides and healthy sides were calculated；the incidence of adverse reactions during treatment was observed； the clinical effects in the two groups were evaluated. Results： After treatment， the total effective rate in the observation group was higher than that in the control group (P＜0.05). The scores of FMA and MBI in the two groups were higher than those before treatment (P＜0.05)， and the above two scores in the observation group were higher than those in the control group (P＜0.05). The RMS ratios of biceps brachii and triceps brachii on the affected side and those on the healthy side in the two groups were higher than those before treatment (P＜0.05)，and the two ratios in the observation group were higher than those in the control group (P＜0.05). During the treatment，there were no obvious adverse reactions in the two groups. Conclusion： The combination use of Jin's three-needle therapy and VR technology can effectively improve the upper limb motor function of patients with stroke complicated with hemiplegia based on routine therapy，and enhance their activities of daily living，with great safety.