Abstract： Objective： To observe the clinical effect of the combination use of proved prescription and acupotomy on lumbar disc herniation. Methods：A total of 82 patients with lumbar disc herniation who were given treatment from December 2022 to October 2023 were screened. They were divided into the control group and the observation group by the random number table method， with 41 cases in each group. The control group was treated with acupotomy；the observation group was treated with acupotomy combined with self-made proved prescription. Both groups were observed for four weeks. The scores of Visual Analogue Scale (VAS) of pain and Japanese Orthopaedic Association (JOA)，lumbar range of motion，and the levels of serum high mobility group protein B1 (HMGB1)，interleukin-1β (IL-1β) and nuclear factor-κB (NF-κB) were compared between the two groups. Results：After two weeks and four weeks of treatment， the VAS scores of pain in the two groups were significantly lower than those before treatment，and the VAS score of pain in the observation group was lower than that in the control group at the same time， differences being significant (P＜0.05). After two weeks and four weeks of treatment， the JOA scores in the two groups were higher than those before treatment，and the JOA score in the observation group was higher than that in the control group at the same time， differences being significant (P＜0.05). After four weeks of treatment，the indexes of lumbar range of motion in the two groups were significantly higher than those before treatment， and the indexes of lumbar range of motion in the observation group were higher than those in the control group，differences being significant (P＜0.05). After four weeks of treatment，the levels of serum HMGB1，IL-1β and NF- κB in the two groups were significantly reduced when compared with those before treatment，and the above three levels in the observation group were lower than those in the control group，differences being significant (P＜0.05). After four weeks of treatment，the total effective rate was 97.56%in the observation group， higher than that of 80.49% in the control group， differences being significant (P＜0.05). Conclusion： The combination use of proved prescription and acupotomy can reduce the levels of serum HMGB1， IL-1β and NF- κB of patients with lumbar disc herniation， mitigate the pain，and improve the lumbar function with great clinical effect.