Abstract： Objective： To observe the effect of Rhei Radix et Rhizoma processed with pork intestines (referred to as intestine-processed Rhei Radix et Rhizoma) on the fasting blood glucose in mice model of constipation， and to preliminarily explore the mechanism of its hypoglycemic effect. Methods： A total of 50 Kunming mice were randomly divided into the normal group with 10 mice and the model establishment group with 40 mice. The model establishment group was given 8 mg/(kg·d) of Loperamide suspension by gavage for 4 consecutive days to establish the mice model of constipation. The 32 successfully modeled mice were then randomly divided into four groups：the model group，the intestine-processed Rhei Radix et Rhizoma group，the lactulose group，and the Rhei Radix et Rhizoma group，each consisting of 8 mice. Additionally，8 mice were randomly selected from the normal group to serve as the control group. Starting from the 5th day，the model group，the intestine-processed Rhei Radix et Rhizoma group，the lactulose group， and the Rhei Radix et Rhizoma group were still given 8 mg/(kg·d) of Loperamide suspension by gavage for 4 consecutive days. Two hours after each gavage， the intestine-processed Rhei Radix et Rhizoma group and the Rhei Radix et Rhizoma group were given their respective suspensions of intestineprocessed Rhei Radix et Rhizoma or Rhei Radix et Rhizoma at a dosage of 0.6 g/(kg·d) for gastric gavage， while the lactulose group was given lactulose suspension at a dosage of 6 g/(kg·d) for gastric gavage. The normal group and the model group were given distilled water at a dosage of 0.2 mL/10 g for gastric gavage. All groups were continuously treated for 4 days. The general condition of the mice in each group was observed， and the body mass was measured after the last gavage； the fasting blood glucose (FBG) was measured twice after the model was successfully established and 4 days after drug intervention. Results： Compared with the control group， the body mass of the mice in the model group increased (P＜0.05)； compared with the model group， the body mass of the mice in the intestine-processed Rhei Radix et Rhizoma group，the lactulose group and the Rhei Radix et Rhizoma group decreased (P＜0.05)；compared with the intestine-processed Rhei Radix et Rhizoma group，the body mass of the mice in the Rhei Radix et Rhizoma group was reduced (P＜0.05). After intervention，the FBG of the mice in the intestine-processed Rhei Radix et Rhizoma group and the Rhei Radix et Rhizoma group was reduced when compared with that before intervention (P＜0.05)，and the FBG of the mice in the intestine-processed Rhei Radix et Rhizoma group and the Rhei Radix et Rhizoma group was lower than that in the model group (P＜0.05). The incidence of adverse drug reactions in the intestine-processed Rhei Radix et Rhizoma group was significantly lower than that in the Rhei Radix et Rhizoma group，the difference being significant (P＜0.05). Conclusion：Rhei Radix et Rhizoma processed with pork intestines can reduce the blood sugar of constipated mice，alleviate the drastic purgative effect of Rhei Radix et Rhizoma，and achieve the effect of reducing blood sugar while improving constipation，and its mechanism can be related to intestinal flora.