Abstract： Objective： To observe the clinical effect of applying umbilical compress with the Jianpi Wentong Prescription combined with Baixiao moxibustion on children with mesenteric lymphadenitis with the syndrome of cold accumulation and abdominal pain. Methods： A total of 94 children with mesenteric lymphadenitis with the syndrome of cold accumulation and abdominal pain were divided into the control group and treatment group by random number table method， with 47 cases in each group. The control group was given conventional western medicine for treatment， and the treatment group was given umbilical compress with theJianpi Wentong Prescription combined with Baixiao moxibustion on the basis of the control group. The following indexes were compared between the two groups before and after treatment： serum nuclear factor- κB (NF- κB)， interleukin-10 (IL-10)， procalcitonin (PCT)， soluble triggering receptor expressed on myeloid cells-1 (sTREM-1)， endothelin-1 (ET-1)， calcitonin generelated peptide (CGRP)， lactic acid (Lac)， diamine oxidase (DAO)， CD4+ T lymphocyte， CD8+ T lymphocyte， immunoglobulin G (IgG)， immunoglobulin A (IgA)， enterobacterium， staphylococcus， bifidobacterium， lactobacillus and longitudinal and transverse diameter of mesenteric lymph nodes. The disappearance time of following symptoms were compared between the two groups： poor appetite， diarrhea， abdominal pain， fever， nausea and vomiting. The clinical effects in the two groups were also compared. Results：After treatment，the total effective rate in the treatment group was higher than that in the control group (P＜0.05). The levels of serum NF- κB， PCT and sTREM-1 in both groups were decreased when compared with those before treatment (P＜0.05)，and the levels of serum IL-10 in both groups were increased when compared with those before treatment (P＜0.05). The levels of serum NF-κB， PCT and sTREM-1 in the treatment group were lower than those in the control group (P＜0.05)， and the serum IL-10 level in treatment group was higher than that in the control group (P＜0.05). The levels of serum IgG，IgA in both groups were decreased when compared with those before treatment (P＜0.05). The levels of serum IgG，IgA in the treatment group were lower than those in the control group (P＜0.05). The levels of serum CD4+ T lymphocyte in both groups were increased when compared with those before treatment (P＜0.05)， and the levels of serum CD8+ T lymphocyte in both groups were decreased when compared with those before treatment(P＜0.05). The levels of serum CD4+ T lymphocyte in the treatment group was higher than that in the control group (P＜0.05)，and the levels of serum CD8+ T lymphocyte in the treatment group was lower than that in the control group (P＜0.05). The levels of serum DAO， ET-1 and Lac in both groups were decreased when compared with those before treatment(P＜0.05)，and the serum CGRP levels in both groups were increased when compared with those before treatment(P＜0.05). The levels of serum DAO，ET-1 and Lac in the treatment group were lower than those in the control group (P＜ 0.05)，and the CGRP level in the treatment group was higher than that in the control group (P＜0.05). The bacterial counts of enterobacteria and staphylococcus in both groups were decreased when compared with those before treatment(P＜0.05)，and the bacterial counts of intestinal bifidobacterium and lactobacillus in both groups were increased when compared with those before treatment(P＜0.05). The bacterial counts of enterobacteria and staphylococcus in the treatment group were less than those in the control group (P＜ 0.05)， and the bacterial counts of intestinal bifidobacterium and lactobacillus in the treatment group were more than those in the control group (P＜0.05). The longitudinal and transverse diameter of mesenteric lymph nodes in both groups were shortened when compared with those before treatment(P＜0.05). The longitudinal and transverse diameter of mesenteric lymph nodes in the treatment group were shorter than those in the control group (P＜0.05). The disappearance time of poor appetite，diarrhea，abdominal pain， fever，nausea and vomiting in the treatment group were shorter than those in the control group (P＜0.05). Conclusion： In the treatment of children with mesenteric lymphadenitis with the syndrome of cold accumulation and abdominal pain， the use of Jianpi Wentong Prescription applied to the navel， Baixiao moxibustion， and conventional western medicine can effectively reduce inflammatory reactions， improve the intestinal mucosal barrier function and intestinal microbiota of children，enhance their immune function， and improve clinical effects.