Abstract：Objective：To observe the curative effect of the combination use of wrist-ankle acupuncture and modified Shenghua Decoction on subinvolution of uterus after second cesarean section. Methods： A total of 150 patients with subinvolution of uterus after second cesarean section were selected and divided into the control group and the study group according to the random number table method，with 75 cases in each group. The control group was given routine treatment， and the study group was additionally treated with wrist-ankle acupuncture and modified Shenghua Decoction based on the treatment of the control group. The clinical effects， and the changes in uterine involution， the traditional Chinese medicine syndrome scores，the scores of World Health Organization Quality of Life-100 (WHOQOL-100)，and the expression levels of matrix metalloproteinase-9 (MMP-9) of endometrium and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) before and after treatment were compared between the two groups. Results：The total clinical effective rate was 97.33% in the study group and 88.00% in the control group， the difference being significant (P＜0.05). After treatment， the fundus of mothers in the two groups was reduced when compared with that before treatment (P＜0.05)， and the fundus in the study group was lower than that in the control group (P＜0.05). After treatment，the traditional Chinese medicine syndrome scores in the two groups were down-regulated when compared with those before treatment (P＜0.05)， and the above scores in the study group were lower than those in the control group (P＜0.05). After treatment， the expression levels of MMP-9 and TIMP-1 in both groups were dwindled when compared with those before treatment (P＜0.05)，and the above two levels in the study group were lower than those in the control group (P＜0.05). After treatment， the QOL scores of physiology， psychology， independence， social relationships， environment and spiritual prop in both groups were up-regulated when compared with those before treatment (P＜0.05)，and the above six scores in the study group were higher than those in the control group (P＜0.05). Conclusion： The combination use of wrist-ankle acupuncture and modified Shenghua Decoction has a great curative effect on uterine involution after second cesarean section， which can enhance the subinvolution of uterus ability， mitigate symptoms such as abdominal pain and improve the quality of life.