Abstract： Objective： To observe the clinical effect of the therapy of Tongguan Liqiao Four-Step Needling combined with Ditan Huazhuo Liyan Prescription on ischemic post-stroke dysphagia and its effects on the surface electromyography of muscle groups of hyoid bone and the levels of neurotrophic factors. Methods：A total of 126 cases of patients with ischemic post-stroke dysphagia were selected and divided into the control group and the trial group according to the random number table method ， with 63 cases in each group. The two groups were given routine treatment；the control group was additionally treated with Ditan Huazhuo Liyan Prescription based on this， and the trial group was additionally treated with Tongguan Liqiao Four-Step Needling based on the treatment of the control group. The two groups were treated for 8 weeks. The clinical effects，swallowing function，surface electromyography of muscle groups of hyoid bone and the levels of neurotrophic factors were compared between the two groups. Results： After treatment， the total effective rate was 93.65% in the trial group， higher than that of 80.95% in the control group (P＜0.05). The scores of Standardized Swallowing Assessment (SSA) and Water Swallowing Test in the two groups were decreased when compared with those before treatment (P＜0.05)， and the scores of Video Fluoroscopic Swallowing Study (VFSS) were increased when compared with those before treatment (P＜0.05)； the scores of SSA and Water Swallowing Test in the trial group were lower than those in the control group (P＜0.05)，and the VFSS score was higher than that in the control group (P＜ 0.05). The biggest amplitude of suprahyoid muscles and infrahyoid muscles was increased when compared with that before treatment (P＜0.05)， and the time course of suprahyoid muscles and infrahyoid muscles was shortened when compared with that before treatment (P＜0.05)；the biggest amplitude of suprahyoid muscles and infrahyoid muscles in the trial group was higher than that in the control group (P＜0.05)，and the time course of suprahyoid muscles and infrahyoid muscles was shorter than that in the control group (P＜0.05). The levels of serum growth differentiation factor 15 (GDF-15) in the two groups were decreased when compared with those before treatment (P＜0.05)， and the levels of serum central nervous system specific protein β (S-100β)，brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were increased when compared with those before treatment (P＜0.05)； the serum GDF-15 level in the trial group was lower than that in the control group (P＜0.05)，and the levels of serum S-100β，BDNF and NGF were higher than those in the control group (P＜0.05). Conclusion：The therapy of Tongguan Liqiao Four- Step Needling combined with Ditan Huazhuo Liyan Prescription can effectively improve the swallowing function of patients with ischemic post-stroke dysphagia，enhance the nerve conduction velocity of hyoid bone and regulate the levels of neurotrophic factors.