Abstract：Objective：To observe the clinical effect of Yijing Huoxue Prescription for varicocele-induced infertility of kidney deficiency and blood stasis type and its effects on sperm DNA fragmentation. Methods： A total of 120 cases of patients with varicocele-induced infertility were selected and divided into the observation group and the control group according to the random number table method，with 60 cases in each group. The observation group was treated with Yijing Huoxue Prescription，and the control group was treated with Maizhiling Tablets. The levels of semen quality and sperm DNA fragmentation indexes (DFI) were compared between the two groups before and after treatment. Clinical effects were compared between the two groups. Results： After treatment， sperm concentration， sperm forward motion， and proportion of normal sperm morphology in the two groups were increased (P＜0.05)，and the above items in the observation group were higher than those in the control group (P＜0.05). After treatment，sperm DFI in the two groups was decreased (P＜0.05)， and the sperm DFI in the observation group was lower than that in the control group (P＜0.05). The total effective rate was 83.3% in the observation group，higher than that of 61.7% in the control group (P＜0.05). Conclusion： Yijing Huoxue Prescription has good clinical effects on patients with varicocele-induced infertility of kidney deficiency and blood stasis type， and can significantly improve sperm quality，reduce DFI levels，and enhance male reproductive ability.