Abstract： Objective： To observe the effect of low-frequency repetitive transcranial magnetic stimulation (LF-rTMS) combined with staging acupuncture on surface electromyography and rehabilitation in post-stroke patients with hemiplegia. Methods：A total of 106 post-stroke patients with hemiplegia were randomly divided into the LF-rTMS group (37 cases)， the acupuncture group (35 cases) and the combination group (34 cases). The LF-rTMS group was treated with LF-rTMS；the acupuncture group was treated with acupuncture by stages，and the combination group was treated with LF-rTMS combined with staging acupuncture. After 6 weeks of treatment，surface electromyography [root mean square (RMS) and co-contraction rate (CR)]，rehabilitation effect [Upper Fugl-Meyer Motor Function Assessment (U-FMA)， Modified Ashworth Scale (MAS) and Modified Barthel Index (MBI)]，and rehabilitation by Brunnstrom stages were compared between the three groups. Results：After treatment，the RMS of elbow flexor biceps and elbow extension triceps was respectively increased when compared with that before treatment (P＜0.05)， and the CR of elbow flexor biceps and elbow extension triceps was respectively decreased when compared with that before treatment (P＜0.05)； the RMS of elbow flexor biceps and elbow extension triceps in the combination group was respectively higher than that in the LF-rTMS group and that in the acupuncture group (P＜0.05)，and the RMS of elbow flexor biceps and elbow extension triceps in the acupuncture group was higher than that in the LF-rTMS group (P＜0.05)；the CR of elbow flexor biceps and elbow extension triceps in the combination group was respectively lower than that in the LF-rTMS group and that in the acupuncture group (P＜0.05)， and the CR of elbow flexor biceps and elbow extension triceps in the acupuncture group was respectively lower than that in the LF-rTMS group (P＜0.05). After treatment，the proportion of cases in the rehabilitation period (Stage Ⅳ + Ⅴ) in the three groups was increased when compared with that before treatment (P＜0.05)，and the proportion of cases in the combination group was higher than that in the LF-rTMS group and that in the acupuncture group (P＜0.05). After treatment， the U-FMA and MBI scores were increased when compared with those before treatment (P＜0.05)， and the MAS scores were decreased when compared with those before treatment (P＜0.05)；the U-FMA and MBI scores in the combination group were higher than those in the LF-rTMS group and the acupuncture group (P＜0.05)，and the MAS score was lower than that in the LF-rTMS group and that in the acupuncture group (P＜0.05)； the U-FMA and MBI in the acupuncture group were higher than those in the LF-rTMS group (P＜0.05)，and the MAS score was lower than that in the LF-rTMS group (P＜0.05). Conclusion：LF-rTMS combined with staging acupuncture can alleviate the spasticity of upper limbs in post-stroke patients with hemiplegia， improve motor function and daily living ability， which may be related to the regulation of surface electromyography of affected limbs.