Abstract： Objective： To observe the clinical effects of oral administration of Zengye Decoction and iontophoresis for functional constipation (FC) with yin fluid depletion syndrome and its effects on gastrointestinal function. Methods：A total of 87 cases of patients with FC with yin fluid depletion syndrome were selected as the study objects and divided into the observation group and the control group according to the random number table method，with 44 and 43 cases in each group respectively. The control group was treated with Lactulose Oral Liquid， and the observation group was treated with oral administration of Zengye Decoction and iontophoresis. Clinical effects，traditional Chinese medicine (TCM)syndrome scores， the improvement of gastrointestinal function serological indicators [motilin (MTL)， D-lactic acid (D-LA)， gastrin (GAS)， and vasoactive intestinal peptide (VIP)]， and the incidence of adverse reactions were compared between the two groups. Results：The total effective rate was 97.73% in the observation group and 86.05% in the control group， the difference being significant (P＜0.05). After treatment， the main symptom scores， the secondary symptom scores， and the total scores of TCM syndromes in the two groups were decreased when compared with those before treatment (P＜0.05)， and the above scores in the observation group were lower than those in the control group (P＜0.05). After treatment，the levels of MTL in serum in the two groups were increased when compared with those before treatment (P＜0.05)， and the levels of D-LA， GAS， and VIP in serum were decreased when compared with those before treatment (P＜0.05)；the MTL level in the observation group was higher than that in the control group (P＜ 0.05)，and the levels of D-LA，GAS，and VIP in serum in the observation group were lower than those in the control group (P＜0.05). During treatment， the total incidence of adverse reactions was 4.55% in the observation group and 18.60% in the control group，the difference being significant (P＜0.05). Conclusion： Oral administration of Zengye Decoction and iontophoresis for FC with yin fluid depletion syndrome can help alleviate the constipation symptom and improve gastrointestinal function， whose curative effects and safety are better than those of western medicine routine treatment.