Abstract： Objective： To observe the clinical effect of the therapy of oral administration of Changyangning Syrup combined with enema with Berberine on acute bacillary dysentery in children. Methods： A total of 142 children with acute bacillary dysentery were selected as the study subjects and divided into the control group and the trial group according to the random number table method ， with 71 cases in each group. The control group was given enema with Berberine， and the trial group was additionally treated with Changyanning Syrup based on the treatment of the control group. The clinical symptoms， signs， control time， clinical effects， incidence of adverse reactions， and the levels of hypersensitive C-reactive protein (hs-CRP)，tumor necrosis factor-α (TNF-α)，alanine aminotransferase (ALT)，blood urea nitrogen (BUN) and quality of life were compared before and after treatment between the two groups. Results： After treatment， the recovery time of body temperature and bowel sound back to normal and disappear time of abdominal pain in the trial group were respectively shorter than those in the control group (P＜0.05). After treatment，the levels of hs-CRP，TNF-α，ALT and BUN in the two groups were decreased (P＜0.05)， and the above levels in the trial group were lower than those in the control group (P＜0.05). After treatment，each score in Rhinoconjunctivits Quality of Life Questionnaire (rhinRQLQ) in the two groups was increased (P＜0.05)，and each score in the trial group was higher than that in the control group (P＜0.05). The total effective rate was 97.18% in the trial group，higher than that of 87.32% in the control group (P＜0.05). The incidence of adverse reactions was 1.41% in the trial group， lower than that of 9.86% in the control group (P＜0.05). Conclusion： The therapy of oral administration of Changyangning Syrup combined with enema with Berberine for acute bacillary dysentery in children can effectively inhibit the levels of serum hs-CRP and TNF-α，and improve the levels of ALT and BUN，which can help the recovery of clinical symptoms with less adverse reactions.