Abstract： Objective： To observe the clinical effect of the therapy of acupuncture combined with modified Anshen Dingzhi Decoction on insomnia with heart- gallbladder qi deficiency syndrome. Methods： A total of 94 patients with insomnia of heart- gallbladder qi deficiency syndrome were divided into the observation group and the control group according to the random number table method，with 47 cases in each group. The control group was given modified Anshen Dingzhi Decoction for treatment， and the observation group was additionally treated with acupuncture based on the treatment of the control group. The two groups were treated for 4 weeks. The traditional Chinese medicine (TCM) syndrome scores，sleep quality，scores of anxiety and depression and levels of neurotransmitters were compared before and after treatment between the two groups； the clinical effects and treatment safety in the two groups were evaluated. Results： The total effective rate was 91.49% in the observation group， higher than that of 76.60% in the control group (P＜0.05). After treatment， the scores of Pittsburgh Sleep Quality Indexes (PSQI)，Self- Rating Scale of Sleep (SRSS)，Self- Rating Anxiety Scale (SAS) and Self- Rating Depression Scale (SDS)，TCM syndrome scores and levels of dopamine (DA) in the two groups were decreased when compared with those before treatment (P＜0.05)， and the levels of 5- hydroxytryptamine (5- HT) were increased (P＜0.05)；the scores of PSQI，SRSS，SAS and SDS，TCM syndrome scores and the DA level in the observation group were lower than those in the control group (P＜0.05)， and the 5- HT level was higher than that in the control group (P＜0.05). There was no serious adverse reaction in the two groups. Conclusion： The therapy of acupuncture combined with modified Anshen Dingzhi Decoction has a significant curative effect on insomnia with heart- gallbladder qi deficiency syndrome，which can improve the sleep quality of patients，mitigate such negative emotions as anxiety and depression，and balance the levels of neurotransmitters with good safety.