Abstract： Objective： To explore the mechanism of Apocyni Veneti Folium- Uncariae Ramulus Cum Uncis in treating hypertension based on network pharmacology and experimental verification. Methods：The main chemical components and action targets of Apocyni Veneti Folium-Uncariae Ramulus Cum Uncis were screened by Traditional Chinese Medicine Systems Pharmacology Platform (TCMSP). The targets of hypertension were investigated by Gene Cards and Online Mendelian Inheritance in Man (OMIM). The common targets for treating hypertension were obtained by intersecting the action targets of Apocyni Veneti Folium-Uncariae Ramulus Cum Uncis and the disease targets. The common targets were imported into the STRING database to construct the protein- protein interaction (PPI) network. The common targets were imported into the DAVID online database for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis， and AutoDock and PyMOL were used for molecular docking and visualization. Model rats with hypertension were established by two-kidney and one-clip method. The rats in the treatment group were given solution of Apocyni Veneti Folium- Uncariae Ramulus Cum Uncis by gavage for two weeks， and the rats in the model group and the blank group were given 0.9% sodium chloride solution by gavage for two weeks. The systolic blood pressure (SBP)， pathological structure of thoracic aorta and inflammatory indexes were compared between the three groups. Results： After screening， 6 active components of Apocyni Veneti Folium， 33 active components of Uncariae Ramulus Cum Uncis，and 152 targets of Apocyni Veneti Folium-Uncariae Ramulus Cum Uncis in treating hypertension were obtained，including tumor necrosis factor (TNF)-α，interleukin (IL)-6，IL-1β，IL-8，IL-4，IL-1α， IL-2，IL-10，chemokine C-C motif ligand 2 (CCL2) and intercellular cell adhesion molecule-1 (ICAM1). A total of 122 signaling pathways including TNF signaling pathway ， hypoxia-inducible factor- 1 (HIF- 1) signaling pathway and Toll- like receptor (TRL) signaling pathway were obtained by KEGG enrichment analysis. Molecular docking results showed that kaempferol and quercetin were well bound to TNF，CCL2 and IL- 8. The experimental results showed that Apocyni Veneti Folium- Uncariae Ramulus Cum Uncis significantly reduced the blood pressure of rats with hypertension， effectively improved the pathological structure of thoracic aorta，and reduced the levels of serum IL-6，IL-10，IL-1β and TNF-α. Conclusion： Apocyni Veneti Folium-Uncariae Ramulus Cum Uncis can treat hypertension by multi-component，multitarget and multi-pathway.