Abstract： Objective： To observe the effect of thumb-tack Needling for Subcutaneou Needling and point application on traumatic stress， gastrointestinal hormones and immune escape after laparoscopic radical resection of colorectal cancer under general anesthesia. Methods： A total of 80 cases of patients with colorectal cancer were divided into the control group and the trial group according to the random number table method，with 40 cases in each group. All patients underwent laparoscopic radical resection of colorectal cancer under general anesthesia， followed by routine symptomatic treatment. After operation， the control group received point application， and the trial group was additionally treated with thumb-tack Needling for Subcutaneou Needling based on the treatment of the control group. Both groups were treated for 2 weeks. The objective response rates (ORR) and incidence of complications were compared between the two groups. The levels of C-reactive protein (CRP)， cortisol (P)， interleukin-6 (IL-6)， vascular endothelial growth factor (VEGF)，transforming growth factor- β1 (TGF- β1) and variant domain 6 of CD44 gene (CD44v6) were compared between the two groups at 24 hours and 2 weeks after operation. The levels of motilin (MTL) and gastrin (GAS) in serum were compared before and after treatment between the two groups. Results：The ORR was 55.00% in the trial group，higher than that of 32.50% in the control group (P＜ 0.05). Two weeks after operation， the levels of CRP， P and IL-6 in serum in the two groups were decreased (P＜0.05)，and the levels in the trial group were lower than those in the control group (P＜0.05). After treatment，the levels of MTL and GAS in serum in the two groups were decreased (P＜0.05)，and the levels in the trial group were higher than those in the control group (P＜0.05). Two weeks after operation， the levels of VEGF，TGF - β1 and CD44v6 in the two groups were decreased (P＜0.05)，and the levels in the trial group were lower than those in the control group (P＜0.05). The total incidence of complications was 2.50% in the trial group， lower than that of 15.00% in the control group (P＜0.05). Conclusion： thumb-tack Needling for Subcutaneou Needling combined with point application can improve the clinical effects of laparoscopic radical resection of colorectal cancer under general anesthesia， alleviate traumatic stress levels，regulate gastrointestinal function，inhibit tumor immune escape，and at the same time help to reduce the incidence of postoperative complications.