Abstract：Objective：To observe the curative effect of Yinzhihuang Oral Liquid combined with blue light and Live Combined Bacilus Subtilis and Enterococcus Faecium Powder for neonatal jaundice， and to analyze the effect of the combined therapy for liver function in children. Methods ： The clinical data of 100 cases of neonatal jaundice was given retrospective analysis， and the patients were divided into the routine treatment group and the Yinzhihuang treatment group according to different treatment plans，with 50 cases in each group. The routine treatment group was treated with blue light and Live Combined Bacilus Subtilis and Enterococcus Faecium Powder，and the Yinzhihuang treatment group was additionally treated with Yinzhihuang Oral Liquid based on the treatment of the routine treatment group. Both groups were treated for 7 days. The clinical effects，hospitalization time，fecal emptying time，and incidence of adverse reactions were compared between the two groups. The levels of bilirubin and liver function indicators in the two groups were compared before and after treatment. Results：The total effective rate was 96.00% in the Yinzhihuang treatment group， lower than that of 84.00% in the routine treatment group (P＜0.05). After treatment，the levels of direct bilirubin (DBil)，indirect bilirubin (IBil)，and total bilirubin (TBil) in serum in the two groups were decreased (P＜0.05)，and the levels in the Yinzhihuang treatment group were lower than those in the routine treatment group (P＜0.05). After treatment， the levels of aspartate aminotransferase (AST)， alanine aminotransferase (ALT)， and alkaline phosphatase (ALP) in serum in the two groups were decreased (P＜0.05)， and the levels in the Yinzhihuang treatment group were lower than those in the routine treatment group (P＜0.05). The hospitalization time and fecal emptying time in the Yinzhihuang treatment group were shorter than those in the routine treatment group (P＜0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P＞0.05). Conclusion：The application of Yinzhihuang Oral Liquid combined with blue light and Live Combined Bacilus Subtilis and Enterococcus Faecium Powder for neonatal jaundice can improve clinical effects， protect liver function， and reduce bilirubin levels and incidence of adverse reactions.