Abstract： Objective： To observe the clinical effect of Yangxue Bushen Tiaochong Prescription combined with estrogen-progesterone sequential therapy for premature ovarian failure of kidney deficiency and blood stasis type. Methods： A total of 96 patients with premature ovarian failure of kidney deficiency and blood stasis type were divided into the control group and the treatment group according to the random number table method， with 48 cases in each group. The control group was treated with estrogenprogesterone sequential therapy， and the treatment group was additionally treated with Yangxue Bushen Tiaochong Prescription based on the treatment of the control group. Both groups were treated continuously for one month. The traditional Chinese medicine syndrome (TCM) scores， sex hormone indicators， and uterine and ovarian ultrasound detection indicators were compared before and after treatment in the two groups. The clinical effects and incidence of adverse reactions were evaluated in the two groups. Results： The total effective rate was 95.83% in the treatment group，higher than that of 81.25% in the control group (P＜0.05). After treatment，the TCM syndrome scores and levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the two groups were decreased when compared with those before treatment (P＜0.05)， and estradiol (E2) and the sum of three diameters of the uterus， endometrial thickness， and average ovarian volume levels were increased (P＜0.05)； after treatment， the sum of three diameters in uterus， endometrial thickness， average ovarian volume level and E2 level in the treatment group were higher than those in the control group (P＜0.05)，and the TCM syndrome scores，and the levels of FSH and LH were lower than those in the control group (P＜0.05). The incidence of adverse reactions was 2.08% in the treatment group， lower than that of 14.58% in the control group (P＜0.05). Conclusion： The combination of Yangxue Bushen Tiaochong Prescription combined with estrogen-progesterone sequential therapy has a definite curative effect on premature ovarian failure of kidney deficiency and blood stasis type， which can regulate the sex hormone levels in serum，improve uterine and ovarian atrophy，and have high safety.