Abstract：Objective：To observe the clinical effect of Bupi Yichang Pills combined with Sulfasalazine for chronic ulcerative colitis. Methods： A total of 90 cases of chronic ulcerative colitis were randomly divided into the control group and the observation group， with 45 cases in each group according to the random number drawing method. The control group was treated with Sulfasalazine，and the observation group was treated with Bupi Yichang Pills combined with Sulfasalazine. Both groups were treated continuously for 30 days. The levels of neutrophil gelatinase-associated lipocalin (NGAL)，matrix metalloproteinase-9 (MMP- 9)，and inflammatory factors [C-reactive protein (CRP)，tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] were compared between the two groups before and after treatment. The traditional Chinese medicine (TCM) syndrome scores，intestinal mucosal lesion scores，and Mayo activity index scores were compared between two groups. The clinical effects and incidence of adverse reactions were evaluated in the two groups. Results：The total effective rate was 97.78% in the observation group，higher than that of 82.22% in the control group (P＜0.05). After treatment， the TCM syndrome scores， intestinal mucosal lesion scores， and Mayo activity index scores in the two groups were decreased when compared with those before treatment (P＜0.05)，and the above indexes in the observation group were lower than those in the control group (P＜0.05). After treatment，the levels of NGAL，MMP-9，CRP，TNF-α and IL-6 were decreased when compared with those before treatment (P＜0.05)，and the levels of inflammatory factors， NGAL and MMP-9 in the observation group were lower than those in the control group (P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion： The combination of Bupi Yichang Pills combined with Sulfasalazine has a definite curative effect on chronic ulcerative colitis，which can significantly improve clinical symptoms，reduce inflammatory factor levels，and have high safety.