Objective：To observe the clinical effect of I-Ching navel acupuncture with the "Sizhengwei" needling Method (obeying the needling sequence of Xùn- Gèn- Qián- Kūn and Lí- Kǎn- Zhèn- Duì) and the “Leifeng Xiangbo” Needling Method (obeying the needling sequence of Zhèn-Xùn) on the intervention in fatigue- predominant sub- health. Methods： From September 2020 to March 2021， the Center of Preventive Treatment of Guangdong Provincial Hospital of Chinese Medicine recruited 71 patients with fatigue- predominant sub- health. The patients were divided into two groups according to different intervention methods. The Sizhengwei Needling group received intervention with the navel acupuncture with "Sizhengwei" Needling Method，and the Leifeng Xiangbo needling group was intervened with the navel acupuncture with "Leifeng Xiangbo" Needling Method. The changes in the scores of total fatigue，physical fatigue，and mental fatigue in the 14-item Fatigue Scale (FS-14) as well as Visual Analogue Scale (VAS) to evaluate the subjective experience of fatigue were compared between the two groups before and after intervention. Results：After intervention，the scores of total fatigue，physical fatigue，and mental fatigue in FS-14 as well as VAS scores of subjective experience of fatigue in the Sizhengwei Needling group were decreased， differences being significant (P＜0.05). The were significant differences being found in the comparison of the scores of total fatigue， physical fatigue， and mental fatigue in FS- 14 as well as VAS scores of subjective experience of fatigue in the Leifeng Xiangbo Needling group before intervention and after intervention (P＜0.05). After intervention， there were significant differences being found in the comparison of the scores of total fatigue and physical fatigue in FS-14 as well as VAS scores of subjective experience of fatigue between the two groups (P＜0.05)；the comparison of the scores of mental fatigue in FS- 14 between the two groups showed no significance in the difference (P ＞ 0.05). The "Sizhengwei" Needling Method was better than the "Leifeng Xiangbo" Needling Method in the improvement of total fatigue and physical fatigue；in terms of improving mental fatigue，the two needling methods had similar effects. Conclusion：I-Ching navel acupuncture has significant clinical effect on the intervention in fatiguepredominant sub- health. "Sizhengwei" Needling Method is better than the "Leifeng Xiangbo" Needling Method in the improvement of total fatigue and physical fatigue