Abstract： Objective： To observe the clinical effect of Wuling Capsules on insomnia with noninteraction between heart and kidney syndrome and its effect on neuroendocrine function. Methods：A total of 120 patients with insomnia with heart- kidney non- interaction syndrome were selected and divided into the study group and the control group according to the random number table method，with 60 cases in each group. The control group was given the oral administration of Dexzopiclone Tablets for treatment and the study group was treated with Wuling Capsules. The clinical effects，incidence of adverse reactions were compared，traditional Chinese medicine (TCM) syndrome score，Pittsburgh Sleep Quality Index (PSQI)， and levels of serum neurotransmitters， including 5- hydroxytryptamine (5- HT)， brain derived neurotrophic factor (BDNF) and gamma-aminobutyric acid (GABA) were compared between the two groups before and after treatment. Results： The total effective rate was 91.67% in the study group and 73.33% in the control group， the difference being significant (P＜0.05). After treatment， the TCM syndrome scores of vexation and insomnia， difficulty in falling asleep， palpitation and dreaminess， dizziness and tinnitus，soreness and weakness of the waist and the knees，tidal fever and night sweating， vexing heat in the chest， palms and soles and dry throat and less fluids in both groups were decreased when compared with those before treatment (P＜0.05)， and the above scores in the study group were lower than those in the control group (P＜0.05). After treatment， the scores of sleep quality， daytime dysfunction， sleep time， sleep disorder， sleep effeciency， sleep medicine and sleep latency in PSQI scale in the study group were significantly decreased when compared with those before treatment and in the control group (P＜0.05)；the scores of sleep quality，daytime dysfunction，sleep time，sleep disorder and sleep latency in PSQI scale in the control group were decreased when compared with those before treatment (P＜0.05)， and the changes of scores of sleep efficiency and sleep medicine before and after treatment were not significant (P＞0.05). After treatment，the levels of serum 5-HT，BDNF and GABA in both groups were increased when compared with those before treatment (P＜0.05)， and the levels of above indexes in the study group were higher than those in the control group (P＜0.05). During treatment， the incidence of adverse reactions was 8.33% in the study group and 21.67% in the control group， the difference being significant (P＜0.05). Conclusion： Wuling Capsules has a significant curative effect on insomnia with non- interaction between heart and kidney syndrome， which can effectively improve the sleep quality of patients，and adjust the secretory function of neurotransmitters with good safety.