Abstract：Objective：To observe the effect of tripterine inducing apoptosis of H929 human myeloma cells， and analyze the molecular mechanism of inducing apoptosis. Methods： H929 cells that are in the plateau stage after 48 hours of cultivation were taken，and were inoculated into 6-well cell culture plates with 1.0×109/L cell density， which were divided into the control group， the tripterine group (0.5 mg/L， 1.0 mg/L， and 5.0 mg/L). Each concentration of the tripterine group was added with corresponding concentration of tripterine，and the control group was added with equal volume of dimethyl sulfoxide (DMSO) solution. The inhibition rates of H929 cell viability at different concentrations of tripterine groups were compared，and the apoptosis rates of H929 cells in each group and the expression levels of P53，cleaved cysteine aspartic protease-3 (cleaved caspase-3) ， cleaved poly ADP-ribose polymerase-1 (cleaved PARP-1)，bax，and bcl-2 in H929 cell culture were compared among the groups. Results：The inhibition rates of H929 cell viability were increased with the increase of tripterine treatment concentration，and there were significant differences being found in the comparison of the inhibition rates of H929 cell viability in different concentrations of tripterine groups (P＜0.05). The apoptosis rates of H929 cells in different concentrations of tripterine treatment group were higher when compared with that in the control group (P＜ 0.05). The apoptosis rates of H929 cells were increased with the increase of tripterine treatment concentration，and there were significant differences being found in the comparison of the apoptosis rates of H929 cells in different concentrations of tripterine groups (P＜0.05). The expression levels of P53， cleaved caspase-3，cleaved PARP-1 and bax in H929 cell cultures treated with different concentrations of tripterine were relatively high when compared with those in the control group (P＜0.05)，and the expression levels of bcl- 2 were relatively low (P＜0.05). The expression levels of P53，cleaved caspase- 3，cleaved PARP-1，and bax in H929 cell cultures were increased with the increase of tripterine treatment concentrations， and the expression levels of bcl-2 were decreased with the increase of tripterine treatment concentrations. There were significant differences being found in the comparison of expression levels of P53， cleaved caspase-3，cleaved PARP-1，bax，and bcl-2 in H929 cell cultures in different concentrations of tripterine groups (P＜0.05). Conclusion： Tripterine can inhibit the viability and induce apoptosis of H929 human myeloma cells， and has a certain dose effect， which may be related to the mitochondrial apoptosis pathway.