Abstract： The short- term curative effect of acupuncture in the treatment of diseases had been confirmed by numerous clinical studies， and its curative mechanism has also been explained by basic studies， but the mechanism of long- term curative effect of acupuncture was still unclear. With the development of modern science and technology and the improvement of research methods， certain methods and basis were provided for the discussion of the specific mechanism of long-term curative effect of acupuncture. The long- term curative effect of acupuncture depends on the stable recovery after tissue injury， and stem cells were the source of tissue regeneration， and their activation， proliferation and differentiation provide possibilities for regeneration and functional recovery after tissue injury. In addition， maintaining homeostasis of the internal environment was the basis of biological survival，and the dynamic balance was destroyed in pathological state， resulting in a series of disease reactions. Correcting homeostasis imbalance and restoring body homeostasis was an important guarantee for the long- term curative effect of acupuncture. Therefore，from the point of view of acupuncture regulating stem cells to promote repair and regeneration after tissue injury and maintain body homeostasis， the possible mechanism of acupuncture producing long-term curative effect was discussed to provide reference for the study of acupuncture effect.