Abstract：Objective：To observe the clinical effect of cupping following collateral bloodletting therapy combined with Liuwei Dihuang Pills for females with chloasma of kidney yin deficiency type. Methods：A total of 64 cases of females with chloasma of kidney yin deficiency type were selected and divided into the control group and the study group according to the random number table method，with 32 cases in each group. Both groups were treated with Iron and Vitamin C Oral Liquid and Vitamin E Soft Capsules， the control group was additionally treated with Liuwei Dihuang Pills， and the study group was additionally treated with cupping following collateral bloodletting therapy based on the treatment of the control group， both groups were treated for 2 months. The levels of estrogen (E2) and luteinizing hormone (LH) in serum and the scores of skin lesions before and after treatment，and the clinical effects were compared between the two groups. The adverse reactions in the two groups were recorded. Results： After treatment， the levels of E2 and LH in serum in the two groups were decreased when compared with those before treatment (P＜0.05)， and the levels of E2 and LH in serum in the study group were lower than those in the control group (P＜0.05). After treatment for 1 and 2 months， the scores of skin lesions in the two groups were decreased when compared with those before treatment (P＜0.05)，and the scores in the study group were lower than those in the control group at the same period (P＜0.05). The total effective rate was 93.75% in the study group，higher than that of 75.00% in the control group (P＜0.05). During treatment，there was no obvious adverse reaction in the two groups. Conclusion：Cupping following collateral bloodletting therapy combined with Liuwei Dihuang Pills has a good clinical effect in treating females with chloasma of kidney yin deficiency type，and can regulate the levels of estrogen and relieve clinical symptoms with good safety.