Abstract： Objective： To observe the clinical effect of Guben Jiedu Quyu Method combined with chemotherapy for advanced non-small cell lung cancer (NSCLC). Methods：A total of 78 cases of patients with advanced NSCLC were randomly divided into the control group and the study group according to the odd- even number grouping method， with 39 cases in each group. The control group was treated with chemotherapy，and the study group was additionally treated with Guben Jiedu Quyu Method based on the treatment of the control group. The clinical effects and the incidence of adverse reactions were compared between the two groups；the levels of tumor markers and immune factors in serum were compared before and after treatment between the two groups. Results： After treatment， the total effective rate was 69.23% in the study group，higher than that of 46.15% in the control group (P＜0.05). Before treatment， there was no significant difference being found in the comparison of the levels of neuron-specific enolase (NSE)， carcinoembryonic antigen (CEA)， and cancer antigen (CA125) in serum between the two groups (P＞0.05). After treatment，the levels of NSE，CEA，and CA125 in the two groups were decreased (P＜ 0.05)，and the above levels in the study group were lower than those in the control group (P＜0.05). Before treatment，there was no significant difference being found in the comparison of CD3+，CD4+，CD8+，and CD4+/CD8+ between the two groups (P＞0.05). After treatment，CD3+，CD4+，and CD4+/CD8+ in the two groups were increased (P＜0.05)，and CD8+ was decreased (P＜0.05)；CD3+，CD4+，and CD4+/CD8+ in the study group were higher than those in the control group (P＜0.05)，and CD8 + was lower than that in the control group (P＜0.05). During treatment， the incidence of nausea and vomiting， leukopenia， thrombocytopenia， liver damage and other adverse reactions in the study group was significantly lower than that in the control group (P＜0.05). Conclusion： Guben Jiedu Quyu Method combined with chemotherapy for advanced NSCLC can effectively reduce the levels of serum tumor markers and improve the immune function，with high safety and a significant curative effect.