Abstract：Objective：To observe the clinical effect of Jianpi Yiqi Tongluo method combined with point injection for diabetic peripheral neuropathy. Methods： A total of 200 cases of patients with diabetic peripheral neuropathy were selected and divided into the control group and the observation group according to the random number table method， with 100 cases in each group. The control group was given point injection with Danshen Injection， and the observation group was additionally treated with Jianpi Yiqi Tongluo method based on the treatment of the control group. The clinical effects in the two groups were evaluated. Before and after treatment， the levels of serum related factors， nerve conduction velocity， clinical symptom scores and quality of life scores were compared between the two groups. Results：The total effective rate was 98.00% in the observation group， higher than that of 91.00% in the control group (P＜0.05). After treatment，the contents of γ-glutamyl transpeptidase (γ-GGT)，advanced glycation end products (AGEs)，high mobility group protein B1 (HMGB1) in serum，von willebrand factor (vWF)，and endothelial cell specific molecule-1 (ESM-1) in the two groups were decreased when compared with those before treatment (P＜0.05)；the contents of γ-GGT，AGEs，HMGB1，vWF and ESM-1 in the observation group were lower than those in the control group (P ＜ 0.05). After treatment ， the contents of lipoxin A4 (LXA4)，superoxide dismutase (SOD)，γ-aminobutyric acid (γ-Gaba)，nerve growth factor (NGF)，and platelet derived endothelial cell growth factor (PD-ECGF) in the two groups were increased when compared with those before treatment (P＜0.05)；the contents of LXA4，SOD，γ-Gaba，NGF and PD-ECGF in the observation group were higher than those in the control group (P＜0.05). After treatment， motor nerve conduction velocity (MCV) and sensory nerve conduction velocity (SCV) of median nerve and common peroneal nerve in the two groups were increased when compared with those before treatment (P＜0.05)； the MCV and SCV of median nerve and common peroneal nerve in the observation group were higher than those in the control group (P＜0.05). After treatment，Toronto Clinical Scoring System (TCSS) scores in the two groups were decreased when compared with those before treatment (P＜0.05)，and TCSS score in the observation group was lower than that in the control group (P＜0.05)；the Generic Quality of Life Inventory- 74 (GQOLI-74) scores in the two groups were increased when compared with those before treatment (P＜ 0.05)， and the GQOLI- 74 score in the observation group was higher than that in the control group (P＜ 0.05). Conclusion： Jianpi Yiqi Tongluo method combined with point injection for diabetic peripheral neuropathy can reduce the levels of AGEs and HMGB1，inflammation，oxidative stress injury and vascular endothelial cell injury，promote nerve repair，improve the levels of endothelial cell related factors and nerve conduction velocity，relieve clinical symptoms，and enhance the quality of life of patients.