Abstract： Objective： To observe the clinical effect of Yunpi Shengjin Tongbian Decoction combined with routine western medicine therapy for functional constipation (FC) in children and its effect on gastrointestinal hormones in serum. Methods： A total of 74 cases of FC children were divided into the control group and the observation group according to the random number table method，with 37 cases in each group. Both groups were given basic treatment including drinking more water， adjusting eating habits，training defecation habits and conducting a moderate amount of physical activity. The control group was treated with routine western medicine，and the observation group was additionally treated with Yunpi Shengjin Tongbian Decoction based on the treatment of the control group. Both groups were treated for four weeks. The clinical effects were evaluated in the two groups. Before and after treatment， the levels of gastrointestinal hormones in serum，traditional Chinese medicine syndrome scores，and recurrence rates in half-a-year follow-up were compared in the two groups. Results：The total effective rate was 94.59% in the observation group，higher than that of 78.38% in the control group，the difference being significant (P＜0.05). After treatment，the levels of motilin (MTL)，substance P (SP) and neurotensin (NT) in serum in the two groups were increased when compared with those before treatment (P＜0.05)，and the levels of MTL，SP and NT in serum in the observation group were higher than those in the control group (P＜0.05). After treatment，the scores of interval time of defecation，stool traits and defecation difficulties in the two groups were decreased when compared with those before treatment (P＜0.05)，and the scores of interval time of defecation，stool traits and defecation difficulties in the observation group were lower than those in the control group (P＜0.05). The recurrence rate was 17.14% in the observation group，lower than that of 41.38% in the control group (P＜0.05). Conclusion： Yunpi Shengjin Tongbian Decoction combined with western medicine has a definite curative effect in treating FC in children. It can not only improve the clinical symptoms， but also reduce the recurrence rates. Its mechanism may be closely related to its ability to regulate the levels of gastrointestinal hormones and improve gastrointestinal function.