Abstract：Objective：To establish a HPLC method for the determination of ephedrine hydrochloride， pseudoephedrine hydrochloride and amygdalin in Xiaochuan Tablets. Method：The chromatographic column was Yuexu XB- C18 (4.6 mm × 250 mm， 5 μm)， the mobile phase is acetonitrile- 0.2% phosphoric acid aqueous solution (gradient elution)， the flow rate is 1.0 mL/min， the detection wavelength is 210 nm， and the column temperature is 30 ℃ . Results： The mass concentrations of ephedrine hydrochloride， pseudoephedrine hydrochloride and amygdalin were 11.552-57.76 μg/mL (r=0.999 5)，7.664-38.32 μg/mL (r=0.999 6)，32.348-161.74 μg/mL (r=0.999 9). The linear relationship between peak area and the range is good. The average recoveries were 99.6% ， 99.2% and 99.7% ， respectively， with RSDs of 0.35% ， 0.41% and 0.28% (n=6). Conclusion：The method is simple， accurate， reproducible and can be used for the quality control of Xiaochuan Tablets.