Abstract：Objective：To observe the curative effect of Buzhong Zhuzhang Prescription combined with lysine inosite vitamin B12 on growth in preschool children with idiopathic short stature(ISS). Methods：A total of 142 cases of preschool children with ISS who met the selection criteria were collected by method of regression cohort study，and divided into the observation group and the control group according to different treatments. The control group was treated with lysine inosite vitamin B12 oral solution，and the observation group was additionally given the oral administration of Buzhong Zhuzhang Prescription combined with lysine inosite vitamin B12 oral solution. The confounding factors were treated by propensity score matching method. After matching，the changes in height(H)，growth velocity(GV)，height standard deviation scores (HtSDS)，body mass index(BMI) and chronological age/bone age(CA/BA) ratio，as well as levels of serum growth hormone peak(GHP)，serum insulin-like growth factor-1(IGF-1) and insulin-like-factor-bindingprotein 3(IGFBP-3) after 6 and 12 months of treatment were observed，counted and analyzed between the two groups. The safety was analyzed. Results： Before matching， there was significant difference being found in the comparison of H，CA/BA，HtSDS，GHP and IGF-1 between the two groups(P＜0.05). After 12 months of treatment， H in the observation group was higher than that in the control group(P＜0.05). After 6 and 12 months of treatment，GV and HtSDS in the observation group were higher than those in the control group at the same time period(P＜0.05). After 6 and 12 months of treatment， there was no significant difference being found in the comparison of GHP between the two groups(P ＞ 0.05). After 6 months of treatment，IGF-1 level in the observation group was higher than that in the control group at the same time period(P＜0.05). After 12 months of treatment， levels of IGF- 1 and IGFBP3 in the observation group were higher than those in the control group at the same time period， the difference being significant(P＜0.05). During the treatment， both groups of children had no special discomfort. Conclusion： Buzhong Zhuzhang Prescription combined with lysine inosite vitamin B12 can effectively promote growth in preschool children with ISS，which is safe for clinical use.