Abstract：Objective：To observe the effect of quercetin on proliferation，apoptosis and metastasis of cervical cancer cells and discuss its mechanism. Methods：Cervical cancer C- 33A cells were divided into the control group and the 20，40，60 μmol/L quercetin groups. The MTT assay was used to detect the cell proliferation activity；the flow cytometry was used to detect the cycle change and apoptosis rate of cells； the Transwell assay was used to detect the cell transfer rate， and the Western blot was used to detect protein tyrosine kinase 1(JAK1)， protein tyrosine kinase 2(JAK2)， and signal transducer and activator of transcription 3(STAT3). Results： Compared with those in the control group， cell proliferation activity， metastasis rates and the percentage of cells in S phase and G2/M phase were significantly decreased in the 20，40，60 μmol/L quercetin groups(P＜0.05)，and the apoptosis rates and the percentage of cells in G0/ G1 phase were significantly increased(P＜0.05)； with the increase of quercetin concentration， the proliferation activity， metastasis rates and the percentage of cells in S phase and G2/M phase were gradually decreased(P＜0.05)， and the apoptosis rates and the percentage of cells in G0/G1 phase were increased significantly(P＜0.05)， the differences being statistically significant. The protein levels of JAK1， JAK2 and STAT3 in the 20，40，60 μmol/L quercetin group were significantly decreased when compared with those in the control group， and with the increase of quercetin concentration， the protein levels of JAK1，JAK2 and STAT3 were gradually decreased(P＜0.05). Conclusion：Quercetin inhibits the proliferation and metastasis of cervical cancer， induces apoptosis and block cell cycle through JAK- STAT signaling pathway.