Abstract：Objective：To observe the clinical effect of five-note therapy combined with Yi-cupping for low back pain of liver-kidney depletion syndrome. Methods：A total of 80 cases of patients with low back pain of liver-kidney depletion syndrome were divided into the experiment group A，the experiment group B， the experiment group C and the control group， with 20 cases in each group. The control group was treated with basic medication；based on the treatment of the control group，the experiment group A was additionally treated with five- note therapy combined with Yi- cupping； the experiment group B was additionally treated with Yi-cupping therapy，and the experiment group C was additionally treated with fivenote therapy. All groups were treated for two weeks. Changes in the scores of Numerical Rating Scale(NRS) and Hamilton Anxiety Scale(HAMA) before and after treatment as well as clinical effects in the four groups were observed and compared. Results： After treatment， the total effective rate of experiment group A， experiment group B and experiment group C was higher than that of the control group(P＜0.05)；The total effective rate of group A was also higher than that of group B and group C(P＜0.05).The total effective rate in the experiment group A was higher than those in the other three groups(P＜0.05)；the total effective rates in the experiment group B and the experiment group C were higher than that in the control group(P＜0.05)； there was no significant difference being found in the comparison of total effective rates between the experiment group B and the experiment group C(P＞0.05). Before treatment， there was no significant difference being found in the comparison of scores of NRS among the four groups(P＞0.05). After treatment， scores of NRS in the four groups were significantly decreased when compared with those before treatment，differences being significant(P＜0.05)；NRS scores in the experiment group A and the experiment group B were lower than that in the control group(P＜0.05)；NRS score in the experiment group C was lower than that in the control group，but there was no significance in the difference(P＞0.05)；NRS score in the experiment group A was lower than that in the experiment group B(P＜0.05)；NRS scores in the experiment group A and the experiment group B were lower than that in the experiment group C(P＜ 0.05). Before treatment，there was no significant difference being found in the comparison of HAMA scores among the four groups(P＞0.05). After treatment， score of HAMA in the experiment group A was significantly decreased when compared with that before treatment(P＜0.05)；the score in the experiment group A was lower than those in the control group and the experiment group B(P＜0.05)； there was no significant difference being found in the comparison of HAMA scores between the experiment group A and the experiment group C(P＞0.05). There was no significant difference being found in the comparison of HAMA scores in the experiment group B，the experiment group C and the control group before and after treatment and between groups(P＞0.05). Conclusion：Five-note therapy combined with Yi-cupping for low back pain of liver- kidney depletion syndrome can significantly relieve pain and anxiety of patients and improve the clinical effect.