Abstract： Objective： To observe the effect of Angong Niuhuang pills on inflammatory factors， vascular endothelial function and neurological function in patients with acute cerebral infarction(ACI) undergoing intravenous thrombolysis. Methods： A total of 100 cases of ACI patients were selected and divided into the study group and the control group according to the random number table method， with 50 cases in each group. The control group was treated with intravenous thrombolysis with urokinase，and the study group was additionally treated with Angong Niuhuang pills based on the treatment of the control group. Inflammatory factors， vascular endothelial function and neurological function before and after treatment in the two groups were compared；adverse reactions were recorded. Results：After treatment， the levels of hypersensitive C-reactive protein(hs-CRP)，tumor necrosis factor-α(TNF-α)，interleukin-6 (IL- 6) and procalcitonin(PCT) in the two groups were decreased when compared with those before treatment(P＜0.05)； the levels of hs- CRP， TNF- α， IL- 6 and PCT in the study group were lower than those in the control group(P＜0.05). After treatment，the levels of brachial artery flow-mediated dilatation (FMD) and nitric oxide(NO) were increased when compared with those before treatment(P＜0.05)，and the levels of endothelin-1(ET-1) and thromboxane A2(TXA2) were decreased(P＜0.05)；the levels of FMD and NO in the study group were higher than those in the control group(P＜0.05)，and the levels of ET- 1 and TXA2 were lower(P＜0.05). After treatment， the scores of Chinese Stroke Scale(CSS) and National Institutes of Health Stroke Scale(NIHSS) in the two groups were decreased when compared with those before treatment(P＜0.05)， and the modified Barthel Index(MBI) scores were increased(P＜0.05)； the scores of CSS and NIHSS in the study group were lower than those in the control group(P＜0.05)，and the MBI score was higher(P＜0.05). During treatment， there was no obvious adverse reaction in the two groups. Conclusion： The application of Angong Niuhuang pills for ACI patients undergoing intravenous thrombolysis can relieve systemic inflammatory responses，and improve vascular endothelial function and neurological function.