Abstract：Objective：To study the molecular mechanism of Xiaoyu Xiezhuo yin for chronic renal failure (CRF) by network pharmacology. Methods：The TCMSP，chemical database and literature were applied to collect active ingredients and action targets of Xiaoyu Xiezhuo yin. The CRF- related targets were obtained from GeneCards，OMIM，DrugBank and TTD databases，and the intersection of targets of Xiaoyu Xiezhuo yin and CRF-related genes was obtained. The Cytoscape 3.7.2 software was used to establish the “active pharmaceutical ingredients- CRF- targets” network；the intersection targets were imported into STRING database to establish the protein- protein interaction network(PPI)； Metascape online tool was used to conduct GO function enrichment analysis and KEGG pathway enrichment analysis for key targets；Autodock vina and Chimera-1.14 software were used for molecular docking of proteins and active ingredients. Results： A total of 68 main active ingredients and 249 target genes of Xiaoyu Xiezhuo yin were obtained by screening；there were 1，533 CRF-related genes，and 101 intersection targets. The key targets of Xiaoyu Xiezhuo yin for CRF are AKT1 and IL- 6， which were the common main components of Radix Astragali， Radix Achyranthis Bidentatae and Herba Plantaginis， as well as PTGS2， which was the common main component of Radix et Rhizoma Rhei，Radix Astragali，Semen Persicae，Radix Achyranthis Bidentatae and Herba Plantaginis. KEGG enrichment analysis screened out 185 pathways including HIF- 1 signaling pathway， PI3K/Akt signaling pathway，NF-κB signaling pathway and JAK-STAT signaling pathway；biological processes mainly involved in oxidative stress response， apoptosis， active oxygen metabolism， etc. The results of molecular docking showed that the key compounds in Xiaoyu Xiezhuo yin，such as quercetin，luteolin and aloe- emodin ， had a good binding ability to IL- 6 ， PTGS2 and other key targets. Conclusion ： The mechanism of Xiaoyu Xiezhuo yin in treating CRF can be through quercetin， luteolin， aloe- emodin and other active ingredients，and through HIF-1，PI3K/Akt，NF-κB，JAK-STAT and other signaling pathways to act on IL-6 and PTGS2 to regulate apoptosis，oxidative stress，lipopolysaccharide reaction，active oxygen metabolism and other biological processes， and finally achieves the effect of delaying the progression of CRF.