Abstract： Objective： To observe the effect of Zibu Ganshen Mingmu tang combined with mecobalamin on intraocular pressure and visual acuity of glaucoma. Methods：A total of 80 patients with glaucoma who met the inclusion criteria were randomly divided into two groups，with 40 cases in each group. There were 80 eyes involved in the study. The control group was treated with mecobalamin， and the observation group was treated with Zibu Ganshen Mingmu tang combined with mecobalamin. The clinical effects，intraocular pressure，visual acuity，vison，amplitude of pattern visual evoked potential(PVEP)， incubation period of P- VEP and the contents of C- reactive protein(CRP) and interleukin- 6(IL- 6) were compared between the two groups. Results：In the end，a total of 40 cases(40 eyes) were included in the observation group，and 38 cases(38 eyes) in the control group. After treatment， the intraocular pressure in the two groups was decreased when compared with that before treatment， and the visual acuity and vision were increased； after treatment， the intraocular pressure in the observation group was lower than that in the control group at the same period，and the visual acuity and vision in the observation group were increased，differences being significant(P＜0.05). After treatment，the levels of CRP and IL-6 in the two groups were decreased when compared with those before treatment，and the above two levels in the observation group were lower than those in the control group，differences being significant(P＜0.05). After treatment，the amplitude of P-VEP in the two groups was increased when compared with that before treatment，and the incubation period of P-VEP was shortened，and the amplitude of P-VEP in the observation group after treatment was higher than that in the control group at the same period，and the incubation period of P-VEP was lower，differences being significant(P＜0.05). The total effective rate was 95.00% in the observation group，and was 80.00% in the control group，the difference being significant(P＜0.05). Conclusion： The therapy of Zibu Ganshen Mingmu tang combined with mecobalamin for glaucoma can improve the intraocular pressure， visual acuity and vision， reduce the content of inflammatory factors and regulate the conduction function of optic nerve.