Abstract： Objective： To observe the clinical effect of the therapy of Danhong injection assisting thrombolytic with recombinant tissue plasminogen activator on the treatment of acute cerebral infarction. Methods：A total of 60 patients with acute cerebral infarction were selected and divided into the study group and the control group by random number table method， with 30 cases in each group. The study group was given thrombolytic with recombinant tissue plasminogen activator，and the observation group was additionally given Danhong injection based on the treatment of the control group. The scores of National Institute of Health Stroke Scale(NIHSS) and Barthel Index were compared between the two groups before and after treatment，and the levels of blood coagulation function were detected. Results：Before treatment，there was no significant difference in the comparison of the NIHSS score between the two groups(P＞0.05). The NIHSS score in the study group was lower than that in the control group 24 hours， 7 days and 14 days after treatment， differences being significant(P＜0.05). Before treatment， there was no significant difference in the comparison of the score of Barthel Index between the two groups(P＞0.05). The score of Barthel Index in the study group was higher than that in the control group， differences being significant(P＜0.05). Before treatment，there was no significant difference in the comparison of the levels of FIB，D-D，APTT and PT between the two groups(P＞0.05). Twenty four hours after treatment，the levels of FIB in the two groups were decreased when compared with those before treatment，and the FIB level in the study group was lower than that in the control group(P＜0.05)；the levels of D-D，APTT and PT in the two groups were increased when compared with those before treatment，and the above three levels in the study group were higher than those in the control group(P＜0.05). Conclusion：The therapy of Danhong injection assisting thrombolytic with recombinant tissue plasminogen activator for acute cerebral infarction can restore neurological function and reduce vascular reocclusion and ischemia reperfusion injury.