人参皂苷Rg1 抑制滑膜细胞样成纤维细胞侵袭增殖及炎性因子释放的实验研究
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R593.22

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苏州市姑苏区卫生人才培养项目(GSWS2020086)


Experimental Study of Ginsenoside Rg1 Inhibits the Invasion and Proliferation of Fibroblast-Like Synoviocytes and the Release of Inflammatory Factors
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    摘要:

    目的:探讨人参皂苷Rg1 对滑膜细胞样成纤维细胞(FLSs) 增殖、侵袭和炎症因子分泌的影响,为临床治疗类风湿性关节炎(RA) 提供理论及实验依据。方法:体外分离培养RA 的FLSs。以肿瘤坏死因子-α(TNF-α) 刺激FLSs,在上述条件中加入不同浓度Rg1(0,0.4,4 及40 mmol/L),培养24 h 后,分别用实时荧光定量PCR(RT-PCR),免疫印迹实验(Westernblot) 和酶联免疫吸附试验(ELISA) 检测白细胞介素-6(IL-6) 和白细胞介素-1β(IL-1β) 的表达变化。CCK-8 克隆形成以及Transwell 评估细胞增殖和侵袭能力。Western-blot 评估Rg1 对丝裂原活化蛋白激酶/细胞外调节蛋白激酶(MAPK/ERK) 通路激活的影响。结果:在基因和蛋白水平,Rg1 能抑制由TNF-α 诱导FLSs 的IL-6 和IL-1β 的表达,并且限制二者的分泌;此外,Rg1能够显著抑制FLSs 的侵袭与增殖;机制上,Rg1 抑制了ERK 的磷酸化,阻碍了MAPK/ERK 通路的激活。结论:Rg1 抑制FLSs中MAPK/ERK 通路的激活,限制细胞的增殖和侵袭,并阻止炎性因子IL-6 和IL-1β 的释放,从而在RA 的治疗过程中发挥作用。

    Abstract:

    Abstract: Objective: To investigate the effects of Ginsenoside Rg1 on the proliferation, invasion and secretion of inflammatory factors of fibroblast-like synoviocytes(FLSs),so as to provide theoretical and experimental basis for the clinical treatment of rheumatoid arthritis(RA). Methods: FLSs of RA were isolated and cultured in vitro. FLSs were stimulated by tumor necrosis factor- α(TNF- α). Different concentrations of Rg1(0, 0.4, 4 and 40 mmol/L) were added in the above conditions. After 24 hours of culture,expression changes of interleukin-6(IL-6) and interleukin-1β(IL-1β) were detected by real-time fluorescence quantitative PCR(RT-PCR),Western blot and Enzyme-linked immunosorbent assay(ELISA). CCK- 8 clone formation and Transwell evaluation of cell proliferation and invasion. The effect of Rg1 on the activation of mitogen activated protein kinase/extracellular regulated protein kinase(MAPK / ERK) pathway was evaluated by Western blot. Results: At the gene and protein levels, Rg1 could inhibit the expressions of IL- 1β and IL- 6 induced by TNF- α, and limit their secretion. In addition, Rg1 can significantly inhibit the invasion and proliferation of FLSs.In mechanism, Rg1 inhibited the phosphorylation of ERK and hindered the activation of MAPK/ERK pathway. Conclusion:Rg1 inhibits the activation of MAPK/ ERK pathway in FLSs,limits cell proliferation and invasion,and prevents inflammatory factors IL-6 and IL-1β. And thus play a role in the treatment of RA.

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刘喆,夏楠楠,刘农军,郝冬林,李宁宁.人参皂苷Rg1 抑制滑膜细胞样成纤维细胞侵袭增殖及炎性因子释放的实验研究[J].新中医,2022,54(11):1-6

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  • 在线发布日期: 2022-06-10
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