Abstract：Objective：To investigate the effect of apigenin on regulation of Sonic Hedgehog signaling pathway and selfrenewal capacity of pancreatic cancer BxPC-3 cells. Methods：Different concentrations of apigenin(10，20，and 50 μmol/L) were used to intervene in pancreatic cancer cells. The proportion of the pancreatic cancer stem cell， CD24 + CD44 + ， was detected by the method of flow cytometry. The expressions of SHH， PTCH1， SMO and GLI1 in the Sonic Hedgehog signaling pathway at mRNA and protein levels were detected by the method of RT-PCR and Western blot. The self-renewal ability of pancreatic cancer cells was observed by the sphere formation assay with serum-free culture. Results：After BxPC-3 cells were intervened with different concentrations of apigenin(10，20 and 50 μmol/L) for 48 hours，the proportion of CD24+ CD44+，the pancreatic cancer cell，was decreased in varying degrees(P＜0.01). Compared with the control group，20 and 50 μmol/L of apigenin significantly decreased the proportion of pancreatic cancer stem cells(P＜0.01)， and significantly inhibited the expression of SHH， PTCH1， SMO and GLI1 at mRNA and protein levels(P＜0.01). After the BxPC- 3 cells in serum-free medium were treated with different concentrations of apigenin(20 and 50 μmol/L) for seven days，the number of tumor stem cell spheres was significantly reduced when compared with that in the control group(P＜0.01). Conclusion： Apigenin inhibiting the self-renewal of pancreatic cancer cells is related to the down-regulation of Sonic Hedgehog signaling pathway.