芹菜素对胰腺癌BxPC-3细胞Sonic Hedgehog信号通路调控和自我更新能力的影响
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R735.9

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杭州市科技计划引导项目(20171226Y162)


Effect of Apigenin on Sonic Hedgehog Signaling Pathway Regulation and Self- Renewal Ability in Pancreatic Cancer BxPC-3 Cells
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    摘要:

    目的:探讨芹菜素对胰腺癌BxPC-3 细胞Sonic Hedgehog 信号通路的调控作用和自我更新能力的影响。方法:用不同浓度(10、20 和50 μmol/L) 芹菜素干预胰腺癌BxPC-3 细胞。应用流式细胞仪法检测胰腺癌肿瘤干细胞(CD24+CD44+) 比例,利用RT-PCR 和Western blot 法检测Sonic Hedgehog 信号通路成员SHH、PTCH1、SMO 和GLI1 在mRNA 和蛋白水平的表达情况,采用无血清培养成球实验观察胰腺癌细胞自我更新能力。结果:BxPC-3 细胞经不同浓度(10、20 和50 μmol/L) 芹菜素干预48 h 后,胰腺癌CD24+CD44+细胞比例均呈不同程度的下降(P<0.01)。与对照组比较,20、50 μmol/L 芹菜素可显著下调胰腺癌肿瘤干细胞比例(P<0.01),且显著抑制Hedgehog 信号通路成员SHH、PTCH1、SMO 和GLI1 在mRNA 和蛋白水平的表达(P<0.01)。无血清培养的BxPC-3 细胞经不同浓度(20 和50 μmol/L) 芹菜素干预7 d 后,肿瘤干细胞成球数目较对照组显著减少(P<0.01)。结论:芹菜素抑制胰腺癌细胞的自我更新能力与下调Sonic Hedgehog 信号通路有关。

    Abstract:

    Abstract:Objective:To investigate the effect of apigenin on regulation of Sonic Hedgehog signaling pathway and selfrenewal capacity of pancreatic cancer BxPC-3 cells. Methods:Different concentrations of apigenin(10,20,and 50 μmol/L) were used to intervene in pancreatic cancer cells. The proportion of the pancreatic cancer stem cell, CD24 + CD44 + , was detected by the method of flow cytometry. The expressions of SHH, PTCH1, SMO and GLI1 in the Sonic Hedgehog signaling pathway at mRNA and protein levels were detected by the method of RT-PCR and Western blot. The self-renewal ability of pancreatic cancer cells was observed by the sphere formation assay with serum-free culture. Results:After BxPC-3 cells were intervened with different concentrations of apigenin(10,20 and 50 μmol/L) for 48 hours,the proportion of CD24+ CD44+,the pancreatic cancer cell,was decreased in varying degrees(P<0.01). Compared with the control group,20 and 50 μmol/L of apigenin significantly decreased the proportion of pancreatic cancer stem cells(P<0.01), and significantly inhibited the expression of SHH, PTCH1, SMO and GLI1 at mRNA and protein levels(P<0.01). After the BxPC- 3 cells in serum-free medium were treated with different concentrations of apigenin(20 and 50 μmol/L) for seven days,the number of tumor stem cell spheres was significantly reduced when compared with that in the control group(P<0.01). Conclusion: Apigenin inhibiting the self-renewal of pancreatic cancer cells is related to the down-regulation of Sonic Hedgehog signaling pathway.

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潘岩,刘鲁明,花永强,楼银妹.芹菜素对胰腺癌BxPC-3细胞Sonic Hedgehog信号通路调控和自我更新能力的影响[J].新中医,2022,54(9):134-138

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  • 在线发布日期: 2022-05-09
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