Abstract：Objective：To observe the effect of tuina manipulation at different intervening time for limb disorder in stroke patients through a multicenter， randomized and controlled clinical study. Methods： A total of 120 cases of stroke patients with limb disorder， who were treated in Acupuncture- Moxibustion and Tuina Rehabilitation Department of Hangzhou First People's Hospital， the Third Affiliated Hospital of Zhejiang Traditional Chinese Medicine University and Yuyao Hospital of Traditional Chinese Medicine， were selected and divided into two groups according to the random number table method， with 60 cases in each group. The observation group was treated with tuina manipulation from the 7th to 14th days after diagnosis；the control group was treated with tuina manipulation from the 14th to 30th days after diagnosis. Before treatment and after eight-week treatment，the scores of Neurological Function Defect Scale(NFDS)，Berg Balance Scale(BBS)，10 m maximum walking speed(10 m MWS)， Fugl- Meyer Assessment(FMA) and Modified Barthel Index(MBI) were compared， and the effect of tuina manipulation at different intervening time for limb disorder in stroke patients was evaluated. Results： After treatment， the scores of NFDS， BBS， 10 m MWS， FMA and MBI in the two groups were significantly improved when compared with those before treatment，differences being significant(P＜0.05). After treatment，the NFDS score in the observation group was significantly decreased when compared with that in the control group， the difference being significant(P＜0.05)； the scores of BBS， 10 m MWS，FMA and MBI in the observation group were significantly increased when compared with those in the control group，differences being significant(P＜0.05). Conclusion：In the early 7th to 14th days，tuina intervention for stroke patients with limb disorder can not only promote their nerve recovery，but also improve walking ability，balance function and motor ability，and effectively improve the ability of daily living.