Abstract：Objective：To study the pharmacological action molecular mechanism of Pudilan Xiaoyan oral solution and its relationship with diseases based on network pharmacology. Methods：The chemical components of Pudilan Xiaoyan oral liquid were searched by published literature. The effective components were screened by SwissADEM website and all predicted targets with probability larger than zero were found in Swisstarget prediction website. The protein- protein interaction(PPI) network of all targets was constructed in String website. The PPI visualization network was constructed by Cytoscape 3.8.0 software. The core targets of Pudilan Xiaoyan oral solution were screened by CytoNCA APP. The enrichment analysis and visual analysis related to diseases were conducted to all targets. Results：A total of 42 active components and 624 targets were screened. The core targets included：RAC-alpha serine/threonine-protein kinase(AKT1)，glyceraldehyde-3-phosphate dehydrogenase(GAPDH)，mitogen-activated protein kinase 3(MAPK3)，albumin(ALB)，caspase 3(CASP3)，Proto-oncogene tyrosine- protein kinase Src(SRC)， epidermal growth factor receptor(EGFR)， mitogen- activated protein kinase 1(MAPK1)， signal transducer and activator of transcription- 3(STAT-3) and tumor necrosis factor(TNF). Enrichment analysis showed that these targets mainly involved in biological processes，including the response to nitrides and toxic substances，trans-synaptic transduction of signal， cascade regulation of mitogen- activated protein kinase(MAPK) and so on. The results of diseaserelated visual analysis showed that these targets were mainly enriched in laboratory diabetes， neurological diseases and tumor diseases. Conclusion： Pudilan Xiaoyan oral solution whose core targets are screened by network pharmacology has pharmacological effects on anti- inflammation， promoting the differentiation of tissue cells and antitumor through core targets，and has potential therapeutic effects on nervous system and tumor diseases.