Abstract：Objective：To study the role of water regulatory protein(AQP1) in the inhibitory effect of Omphalia lapidescens protein on the migration of gastric cancer cell line SGC- 7901. Methods： SGC- 7901 cells were intervened with three concentrations of Omphalia lapidescens protein pPeOp at 30 μg/mL、60 μg/mL、90 μg/mL. 5-fluorouracil(5-Fu) was used as positive control，acetazolamide and carbachol were used as AQP1 agonists and inhibitors. The effect of pPeOp on the activity of SGC-7901 cells was detected by MTS(3-(4，5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H- tetrazolium). Cell migration was detected by scratch method.The expression of AQP1 protein was detected by Western blot. Results： The cell survival rate in the dosing group was lower than that in the normal group， which was negatively correlated with the concentration of pPeOp(P＜0.05).There was no significant difference in cell survival between acetazolamide and carbachol. The results of scratch method showed that the migration ability of gastric cancer cells decreased with the increase of pPeOp concentration；Western blot showed that the expression of AQP1 decreased in pPeOp treatment group. The expression of AQP1 decreased after high concentration acetazolamide. The expression of AQP1 increased after higher concentration of carbachol. Conclusion：Omphalia lapidescens protein pPeOp can significantly inhibit the expression and migration of aquaporin AQP1 in gastric cancer SGC-7901 cells.