Abstract：Objective：To investigate the effects of Zhizhu tang on neural function and the expression of neurite outgrowth inhibitor-A(Nogo-A) and lysophosphatidic acid(LPA) in hippocampus of rats with acute cerebral infarction(ACI). Methods：The rat model of ACI was prepared by thread thrombus method. The successful rats were randomly divided into model group，low (6.9 g/kg)， medium(13.8 g/kg) and high(27.6 g/kg) dose groups of Zhizhu tang， with 14 rats in each group. 14 rats (only isolated blood vessels without blocking blood flow) were taken as the sham operation group. The low，medium and high dose groups of Zhizhu tang were gavaged with 6.9 g/kg，13.8 g/kg and 27.6 g/kg of Zhizhu tang respectively. The sham operation group and model group were gavaged with equal volume of 0.9% sodium chloride solution. After continuous administration for 14 days，the neurological deficit score，the content of serum lactate dehydrogenase(LDH) and glutathione peroxidase(GSHPx)， the volume proportion of cerebral infarction， the brain water content and Nogo- A and LPA protein levels in hippocampus. Results： Compared with the sham operation group， the neurological deficit score， brain water content， cerebral infarction volume ratio，serum LDH，Nogo-A and LPA protein levels in hippocampus were significantly increased， and the content of serum GSH-Px was significantly decreased in the model group(P＜0.05).Compared with the model group， the neurological deficit score，brain water content，cerebral infarction volume ratio，serum LDH，Nogo-A and LPA protein levels in hippocampus decreased， and the content of serum GSH- Px increased in a dose- dependent manner(P＜0.05). Conclusion：Zhizhu tang can improve neurological function and reduce the volume proportion of cerebral infarction in ACI rats. Its mechanism may be related to Zhizhu tang inhibiting the expression of Nogo-A and LPA protein in hippocampus.