Abstract： Objective： To observe the clinical effect of auricular point application combined with relaxation training for anxiety. Methods：A total of 120 cases of patients with anxiety were divided into the observation group and the control group according to the random number table method， with 60 cases in each group. Both groups were treated with paroxetine hydrochloride；the control group was additionally treated with relaxation training，and the observation group was additionally treated with auricular point application based on the treatment of the control group. Both groups were treated for eight weeks. Sleep quality，anxiety degree and levels of neuropeptide Y(NPY)，cortisol(CS) and prostaglandin E2(PGE2) before and after treatment as well as clinical effects were compared between the two groups. Results：The total effective rate was 95.00% in the observation group，higher than that of 81.67% in the control group(P＜0.05). After treatment，scores of Pittsburgh Sleep Quality Index(PSQI) and Hamilton Anxiety Scale(HAMA) in the two groups were decreased when compared with those before treatment(P＜0.05)，and the two scores in the observation group were lower than those in the control group(P＜0.05). After treatment，levels of NPY，CS and PGE2 in the two groups were decreased when compared with those before treatment(P＜ 0.05)， and the three levels in the observation group were lower than those in the control group(P＜0.05). Conclusion： Auricular point application combined with relaxation training in the treatment of anxiety can relieve anxiety degree of patients， improve their sleep quality and regulate levels of NPY，CS and PGE2.