Abstract：Objective：To observe the clinical effects of the therapy of tuina on meridians and points corresponding to the zang-fu organs based on the theory of spleen-kidney-brain communication combined with rehabilitation training on autism spectrum disorder(ASD). Methods：A total of 104 children with ASD were selected and divided into the tuina group and the rehabilitation group according to the random number table method，with 52 cases in each group. The rehabilitation group was treated with routine rehabilitation training， and the tuina group was additionally treated with tuina on meridians and points corresponding to the zang-fu organs based on the treatment of routine rehabilitation training. Both groups were treated for six courses，four weeks being a course. Before and after treatment，the changes of scores of Autism Behavior Checklist(ABC) and Autism Treatment Evaluation Checklist(ATEC) in the two groups were compared. The clinical effects in the two groups were observed. Results： After treatment， the ABC scores in the two groups were decreased when compared with those before treatment，and the ABC score in the tuina group was lower than that in the rehabilitation group，differences being significant(P＜0.05). After treatment， the total scores and scores of each subscale in ATEC， including speech/language/ communication，social interaction，sensory/perception and health/body/behavior，in the two groups were decreased when compared with those before treatment，and the above scores in the tuina group were lower than those in the rehabilitation group， differences being significant(P＜0.05). After treatment， the total clinical effective rate was 86.54% in the tuina group，and 74.51% in the rehabilitation group，the difference being significant(P＜0.05). Conclusion：The therapy of tuina on meridians and points corresponding to the zang- fu organs combined with rehabilitation training can effectively improve the behavior，language，social，sensory and other functions of children with ASD and alleviate the clinical symptoms of children.