Abstract： Objective： To investigate the effect and molecular mechanism of Actinidia chinensis radix extract on apoptosis，invasion and migration of oral squamous cell carcinoma cells. Methods：Oral squamous cell carcinoma CAL- 27 cells were divided into control group， low， medium and high dose Actinidia chinensis radix groups， high dose Actinidia chinensis radix+ pcDNA group and high dose Actinidia chinensis radix+ pcdna-SBF2-AS1 group. Apoptosis was detected by flow cytometry.Transwell detected cell migration and invasion. Western blot was used to detect the protein expression of E- cadherin， N- cadherin， Cleaved cysteinyl aspartate specific proteinase3(Cleaved- caspase3) and Pro- caspase3. The expression levels of set binding factor 2 antisense RNA1(SBF2-AS1) and miR-329 were detected by real-time fluorescence quantitative PCR(RT-qPCR). Double Luciferase Report experiment verified the targeting relationship between SBF2-AS1 and miR- 329. Results： After treatment with different doses of Actinidia chinensis radix extract， the apoptosis rate of oral squamous cell CAL- 27 increased， the number of migrating and invasive cells decreased ， the expression levels of Ecadherin and Cleaved- caspase3 increased ， the expression levels of N- cadherin and Pro- caspase3 decreased ， the expression level of SBF2-AS1 decreased，and the expression level of miR-329 increased in a dose-dependent manner(P＜ 0.05). Overexpression of SBF2-AS1 could reverse the effects of Actinidia chinensis radix extract on apoptosis，migration and invasion of CAL-27. SBF2-AS1 targets miR-329. Conclusion：Actinidia chinensis radix extract may inhibit the invasion and migration of oral squamous cell carcinoma cells and promote apoptosis by regulating SBF2-AS1/miR-329.