Abstract：Objective：To observe the clinical effect of Shugan Jieyu capsules combined with flupentixol and melitracen tablets for neurasthenia with liver depression and spleen deficiency syndrome and its effects on the levels of dopamine(DA) and 5-hydroxytryptamine(5-HT) in serum. Methods：A total of 66 cases of patients with neurasthenia with liver depression and spleen deficiency syndrome were randomly divided into the control group and the treatment group. Both groups were treated with flupentixol and melitracen tablets，and the treatment group was additionally treated with Shugan Jieyu capsules. Both groups were treated for 12 weeks. Before treatment and after 12 weeks of treatment，the scores of Symptom Checklist 90(SCL- 90) and Hamilton Depression Rating Scale(HAMD- 17)， the symptom scores of liver depression and spleen deficiency syndrome and the levels of DA and 5-HT in serum in the two groups were compared. The clinical effects in the two groups were compared. Results： After treatment， the total effective rate of clinical effect was 93.94% in the treatment group and 69.70% in the control group，the difference being significant(P＜0.05). The scores of SCL-90 and HAMD-17 in the two groups were decreased when compared with those before treatment(P＜0.01)， and the above two scores in the treatment group were lower than those in the control group(P＜0.01). The symptom scores of liver depression and spleen deficiency syndrome in the two groups were decreased when compared with those before treatment(P＜0.01)， and the symptom score in the treatment group was lower than that in the control group(P＜0.01). The levels of DA in serum in the two groups were increased when compared with those before treatment(P＜0.01)， and the levels of 5- HT in serum were decreased(P＜0.01). And the inprovement of the above levels in the treatment group were significant than those in the control group(P＜0.01). Conclusion： The therapy of Shugan Jieyu capsules combined with flupentixol and melitracen tablets for neurasthenia with liver depression and spleen deficiency syndrome has a significant effect， and can help to improve the mental symptoms， depression and Chinese medicine syndromes of patients. This therapy can play a therapeutic role in neurasthenia by regulating the levels of DA and 5-HT.