Abstract：Objective：To observe the clinical effect of Kanglaite injection combined with chemotherapy for gastric cancer and its effect on tumor markers. Methods：A total of 80 cases of patients with gastric cancer were divided into the control group and the observation group according to the random number table method，with 40 cases in each group. The control group was given chemotherapy with paclitaxel， cisplatin and 5- fluorouracil， and the observation group was additionally treated with Kanglaite injection based on the treatment of the control group. After three cycles of chemotherapy，the clinical effects in the two groups were evaluated. Before and after treatment， the levels of carcinoembryonic antigen(CEA)， carbohydrate antigen(CA)199， CA125 and CA724 as well as the incidence of toxic side effects in the two groups were compared. Results： The effective rate was 65.00% in the observation group， higher than that of 40.00% in the control group(P＜0.05). Before treatment，there was no significant difference being found in the comparison of the levels of CEA， CA199， CA125 and CA724 between the two groups(P＞0.05). After treatment， the levels of CEA， CA199， CA125 and CA724 in the two groups were lower than those before treatment(P＜0.05)，and all the indexes in the observation group were lower than those in the control group(P＜0.05). During treatment， there was no significant difference being found in the comparison of the incidences of nausea and vomiting， thrombocytopenia， leukopenia， alopecia， proteinuria and abnormalities in liver and kidney between the two groups(P＞0.05). Conclusion： Kanglaite injection combined with chemotherapy for gastric cancer can obtain a good clinical effect and effectively reduce the levels of tumor markers，without increasing the incidence of adverse reactions.