Abstract： Objectives： To observe the clinical effect of Shengmai injection combined with ultrasound- guided fluid resuscitation for septic shock. Methods：A total of 84 cases of patients with septic shock were divided into the control group and the observation group according to the random number table method，with 42 cases in each group. The control group was given routine symptomatic treatment and ultrasound- guided fluid resuscitation， and the observation group was additionally treated with Shengmai injection based on the treatment of the control group. Before and after treatment， the severity of disease， and indexes of T lymphocyte subsets and hemodynamics in the two groups were compared. The prognosis in the two groups were compared. Results：After treatment，the scores of Sequential Organ Failure Assessment (SOFA)，Acute Physiology and Chronic Health EvaluationⅡ(APACHE Ⅱ) in the two groups were decreased when compared with those before treatment (P＜0.05)，and the scores of SOFA and APACHE Ⅱ in the observation group were lower than those in the control group (P＜0.05). After treatment，the levels of CD3+ and CD4+ in serum in both groups were increased when compared with those before treatment (P＜0.05)，and the CD8+ level in serum was decreased(P＜0.05)；the levels of CD3+ and CD4+ in serum in the observation group were higher than those in the control group(P＜0.05)，and the CD8+ level in serum was lower(P＜0.05). After treatment，the mean arterial pressure(MAP)，central venous pressure(CVP) and systemic vascular resistance index(SVRI) in the two groups were increased when compared with those before treatment(P＜0.05)， and the MAP， CVP and SVRI in the observation group were higher than those in the control group (P＜0.05). The time of mechanical ventilation， vasoactive drug using and intensive care unit (ICU) hospitalization in the observation group were shorter than those in the control group (P＜0.05). Conclusion： Shengmai injection combined with ultrasound- guided fluid resuscitation for septic shock can improve the indexes of T lymphocyte subsets and hemodynamics，and promote disease outcome.