Abstract： Objective： To explore the relationship between various factors and adverse reactions of bee- venom acupuncture therapy. Methods：A questionnaire survey was conducted among 188 randomly selected patients who received bee- venom acupuncture therapy for five weeks. The main contents of the survey included local symptoms， systemic symptoms and signs of adverse reactions，and the factors were analyzed by logistic regression. Results：Adverse reactions of bee- venom acupuncture therapy with high incidence were redness， pain， itching， and skin induration， with the incidence of more than 90%；pigmentation，skin petechia and blisters，and lymphadenopathy were also common，and the occurrence rates were between 26% and 76%；profuse sweating，fatigue，muscle soreness，aversion to cold and fever often occurred at the same time，with the occurrence rate of 15% to 20%. The incidence of abdominal pain and diarrhea was less than 15%；the occurrence rates of rubella and dizziness were less than 5%，and the duration was not long. According to logistic regression analysis，the occurrence of redness and swelling as well as itching was related to the cumulative amount of bee venom；the occurrence of skin induration was related to age，body mass，course of treatment，cumulative amount and frequency；the occurrence of pigmentation was related to the course of treatment，cumulative amount and frequency of bee acupuncture；the occurrence of skin petechia and blisters was related to the therapy，cumulative amount and location； the occurrence of lymphadenopathy was related to the cumulative amount and location of bee acupuncture；the occurrence of aversion to cold and fever was related to the therapy and cumulative amount(P＜0.05). There was no correlation between local pain and the factors listed. Conclusion：The cumulative amount of bee venom is an independent risk factor for adverse reactions of bee-venom acupuncture therapy，and special attention should be paid to clinical operation.