Abstract：Objective：To observe the clinical effect of ginsenoside Rg3 in adjuvant therapy of colon cancer. Methods：A total of 89 cases of patients with stage III colon cancer were divided into the observation group and the control group according to the random number table method，45 and 44 cases in each group. The control group was treated with fluorouracil (5-FU)，calcium folinate，and oxaliplatin，and the observation group was additionally treated with ginsenoside Rg3 based on the treatment of the control group. Short- term curative effect was compared between the two groups. Before and after treatment，the level of immune cells，vascular endothelial growth factor(VEGF)，the expression of p53，and the score of quality of life were compared between the two groups. Results： The short- term curative effect was 82.22% in the observation group， higher than that of 59.09% in the control group， the difference being significant(P＜0.05). Before treatment， when compared levels of interleukin- 2(IL- 2)， interferon- γ(IFN- γ)， tumor necrosis factor- α(TNF- α)， interleukin-4(IL-4)，interleukin-6(IL-6)，and interleukin-10(IL-10) between the two groups，there was no significance in the difference(P＞0.05). After treatment， levels of IL- 2， IFN- γ， and TNF- α in the two groups were higher than those before treatment，and the three levels above in the observation group were higher than those in the control group(P＜0.05)； levels of IL- 4， IL- 6， and IL- 10 in the two groups were lower than those before treatment， and the three levels in the observation group were lower than those in the control group(P＜0.05). Before treatment，there was no significant difference being found in the comparison of levels of VEGF and p53 between the two groups(P＞0.05). After treatment，levels of VEGF and p53 in the two groups were lower than those before treatment，and the two levels in the observation group were lower than those in the control group，the difference being significant(P＜0.05). Before treatment，when compared scores of four domains of quality of life between the two groups， there was no significance being found in the difference(P＞0.05). After treatment， scores of four domains of quality of life in the two groups were increased when compared with those before treatment，and each score in the observation group was higher than that in the control group，the difference being significant(P＜0.05). Conclusion：Ginsenoside Rg3 has a defined curative effect in adjuvant therapy of colon cancer，which can promote the stability of levels of immune cells，regulate levels of VEGF and p53，and improve the quality of life.