Abstract：Objective：To observe the effect of catalpol on the liver function and the indexes related to oxidative stress in mice models with hyperthyroidism，and to discuss the mechanism of catalpol on prevention and treatment of hyperthyroidism complicated with the liver injury. Methods：A total of 40 healthy BALB/c female mice were divided into the blank group，the model group，the methimazole group(the western medicine group)，and the catalpol group，10 mice in each group. Except for the blank group，the models with hyperthyroidism in the other groups were prepared. After successful establishment，the model group and the blank group were given gastric perfusion with distilled water. The western medicine group and the catalpol group were given gastric perfusion with corresponding medicine. After medication， eyeballs were extracted and blood was drawn. Enzyme- linked immunosorbent assay was applied to detect the contents of thyroid stimulating hormone (TSH)，triiodothyronine(T3)，Tetraiodothyronine(T4)，aspartate transaminase(AST)，alanine transaminase(ALT)，and alkaline phosphatase(ALP) in serum. Colorimetry was used to detect activity units of malondialdehyde(MDA) and superoxide dismutase (SOD). Biochemistry was used to detect the content of catalase(CAT). Results：Compared with those in the blank group，the content of TSH in serum in the model group was decreased(P＜0.05)；contents of T3，T4，AST，ALT，and ALP in serum as well as contents of MDA， SOD， and CAT in the liver tissue were increased(P＜0.05). Compared with those in the model group，contents of TSH in serum in the catalpol group and the western medicine group were increased(P＜0.05)；contents of T3，T4，AST，ALT，and ALP in serum as well as contents of MDA，SOD，and CAT in the liver tissue were decreased(P＜ 0.05). Compared with those in the western medicine group，content of TSH in serum in the catalpol group was increased(P＜ 0.05)；contents of T3，T4，AST，ALT，and ALP in serum as well as contents of MDA，SOD，and CAT in the liver tissue were decreased(P＜0.05). Conclusion： Catalpol has a better effect than western medicine in the treatment of hyperthyroidism，which can further relieve oxidative stress injury in the liver.